miltenyi anti cd3 cd28 micro beads (Miltenyi Biotec)
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Miltenyi Biotec
miltenyi anti cd3 cd28 micro beads
Miltenyi Anti Cd3 Cd28 Micro Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+cd28+activation+beads/T+Cell+Activation%2FExpansion+Kit%2C+human/us12606635-838-14-17
Average 97 stars, based on 217 article reviews
Miltenyi Anti Cd3 Cd28 Micro Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+cd28+activation+beads/T+Cell+Activation%2FExpansion+Kit%2C+human/us12606635-838-14-17
Average 97 stars, based on 217 article reviews
miltenyi anti cd3 cd28 micro beads - by Bioz Stars,
2026-09
97/100 stars
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Activation Assay:Article Title: CARD8 inflammasome activation during HIV-1 cell-to-cell transmission Article Snippet: Primary CD4+ T cells were isolated via positive selection using the EasySep Release Human CD4 Positive selection kit (Stem Cell Technologies cat: 17752) according to the manufacturer’s instructions from PBMCs collected from blood donors and seeded at 2.5 × 10 6 cells/mL in the presence of 100 U/mL IL-2. .. T cells were activated 24 hours post-isolation with Article Title: Mediator complex subunit 1 architects a tumorigenic Treg cell program independent of inflammation Article Snippet: RPMI , Cytiva , #SH30255.02. .. Article Title: CARD8 inflammasome activation during HIV-1 cell-to-cell transmission Article Snippet: CD4+ T cell isolation, infection, and coculture Primary CD4+ T cells were isolated via positive selection using the EasySep Release Human CD4 Positive selection kit (Stem Cell Technologies cat: 17752) according to the manufacturer’s instructions from PBMCs collected from blood donors and seeded at 2.5 × 106 cells/mL in the presence of 100 U/ mL IL- 2. .. T cells were activated 24 hours post- isolation with Article Title: Human papillomavirus-encoded circular RNA circE7 promotes immune evasion in head and neck squamous cell carcinoma Article Snippet: Here is the detailed process: We first used the Ficoll method (Cytiva, USA) to isolate peripheral blood mononuclear cells (PBMCs) from the peripheral blood of healthy donors. .. Then, we activated the primary T cells by adding Article Title: Tri-specific antibodies Article Snippet: Human CD8+ T cells were enriched using the EasySepTM Human CD8+ T Cell Enrichment Kit (Stemcell #19053) as per the manufacturers protocol. .. Enriched CD8+ T cells were stimulated using Article Title: Tri-specific antibodies Article Snippet: Human CD4+ T regulatory cells were generated using the EasySepTM Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Stemcell #18063) as per the manufacturers protocol. .. Enriched T regulatory cells were stimulated using Isolation:Article Title: CARD8 inflammasome activation during HIV-1 cell-to-cell transmission Article Snippet: CD4+ T cell isolation, infection, and coculture Primary CD4+ T cells were isolated via positive selection using the EasySep Release Human CD4 Positive selection kit (Stem Cell Technologies cat: 17752) according to the manufacturer’s instructions from PBMCs collected from blood donors and seeded at 2.5 × 106 cells/mL in the presence of 100 U/ mL IL- 2. .. T cells were activated 24 hours post- isolation with Cell Culture:Article Title: Tri-specific antibodies Article Snippet: Human CD8+ T cells were enriched using the EasySepTM Human CD8+ T Cell Enrichment Kit (Stemcell #19053) as per the manufacturers protocol. .. Enriched CD8+ T cells were stimulated using Article Title: Tri-specific antibodies Article Snippet: Human CD4+ T regulatory cells were generated using the EasySepTM Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Stemcell #18063) as per the manufacturers protocol. .. Enriched T regulatory cells were stimulated using Recombinant:Article Title: Tri-specific antibodies Article Snippet: Human CD8+ T cells were enriched using the EasySepTM Human CD8+ T Cell Enrichment Kit (Stemcell #19053) as per the manufacturers protocol. .. Enriched CD8+ T cells were stimulated using Article Title: Tri-specific antibodies Article Snippet: Human CD4+ T regulatory cells were generated using the EasySepTM Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Stemcell #18063) as per the manufacturers protocol. .. Enriched T regulatory cells were stimulated using |
![CAF exosomes directly inhibit T-cell proliferation. A, Schematic of experimental design. B and C, Proliferation of CFSE-labeled murine T cells 72 hours after concomitant stimulation <t>(Stim.)</t> <t>with</t> <t>anti-CD3/CD28</t> antibodies and 10 μg/1 × 10 5 cells cancer cell or CAF exosomes (Exo.) from the MCaP line. Unstimulated (Unstim.) T cells used as a negative control. Dot plot of CD8 T cells shown in B . Proliferation of gated CD8 and CD4 T cells shown in C . D, Representative contour plot of granzyme B+ CD8 + T cells in the presence of anti-CD3/CD28 antibodies and cancer cell or CAF exosomes from the MCaP line for 72 hours. Quantification shown in E . F, Cancer cell or CAF exosomes (10 μg/1 × 10 5 cells) from 4T1 tumor explants were added (10 μg/1 × 10 5 cells) to single-cell suspensions from lymph nodes along with anti-CD3/CD28 antibodies. Proliferation of gated CD8 and CD4 T cells after 72 hours shown. G, Exosomes from human normal fibroblasts (NF) or human luminal CAFs were added (10 μg/1 × 10 5 cells) to single-cell suspensions from human PBMCs concomitantly with anti-human CD3/CD28 antibodies. Proliferation of gated CD8 and CD4 T cells shown. Experiments depict three technical replicates from at least three biological replicates. Significance was tested using one-way ANOVA with Tukey honestly significant difference post hoc test. [ A, Created in BioRender. Jones, D. (2026) https://BioRender.com/nbcfuj2 .]](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2019/pmc13012019/pmc13012019__crc-25-0259_f3.jpg)
![Exosomal ECM from CAFs attenuates T-cell proliferation. A, Mean difference plot showing the log fold change and average abundance of each protein from CAF exosomes. Proteins with fold changes >1 are highlighted [red, up after proteinase K (PK) or blue, down after PK treatment]. Top 10 labeled. B, Gene set enrichment analysis identified proteins associated with ECM organization in CAF exosomes. C, Western blot of COL1A1 and COL5A2 proteins in cells (top) and in exosomes (bottom) of normal fibroblasts (NF) from the MFP of naïve animals and CAFs from the MCaP murine cell line. D, Western blot of COL1A1 and COL5A2 proteins in cells (top) and in exosomes (bottom) of untransformed normal human fibroblasts (NF) and CAFs from luminal breast cancer. E and F, Protein content based on BCA assay ( E ) and extracellular vesicle composition ( F ) of fractions 1–12 collected by iodixanol density gradient centrifugation. G, Western blot of fractions from ultracentrifugation, probed with antibodies against COL1A1, COL5A2, exosome markers Alix and CD63, and the serum protein albumin. H, Western blot of COL1A1 (top) and COL5A2 (bottom) in MCaP CAFs with empty vector (vector) or targeted sgRNA (sg) vectors. I, Exosomes from MCaP CAFs with empty vector or targeted sgRNA constructs were added to CFSE-labeled T cells along with anti-CD3/CD28 antibodies. CFSE dilution in T cells was measured by flow cytometry 72 hours later. J, Western blot of CAF exosomes after collagenase I digestion for 1 hour at indicated concentrations. K, CAF exosomes after digestion with collagenase I at indicated concentration were added to CFSE-labeled T cells along with anti-CD3/CD28 antibodies. CFSE dilution was measured by flow cytometry 72 hours later. Significance was tested using one-way ANOVA with Tukey honestly significant difference post hoc test ( I and K ). L, Naïve CD8 + T cells isolated from Balb/c mice were activated with anti-CD3/CD28 antibodies and nucleofected 72 hours later with Cas9 and sgRNAs targeting SHP2, DDR1, or LAIR-1, or with mock nucleofection (mock). Knockdown efficiency was assessed by Western blot. M, A subset of T cells from each cohort described in ( L ) was labeled with CFSE and subsequently stimulated with CAF-derived exosomes and anti-CD3/CD28 antibodies. Proliferation was assessed by flow cytometry 72 hours later. CFSE-labeled T cells stimulated with anti-CD3/CD28 antibodies alone served as positive controls (Stim.), whereas unstimulated cells (“Unstim.”) served as negative controls. Significance was tested using one-way ANOVA with Tukey honestly significant difference post hoc test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2019/pmc13012019/pmc13012019__crc-25-0259_f5.jpg)
